active myc cdc42 q61l Search Results


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prof gary bokoch  (Addgene inc)
90
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myc cdc42 q61l  (Addgene inc)
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cdc42 q61l  (Santa Cruz Biotechnology)
96
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plasmids 12981  (Addgene inc)
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Addgene inc plasmids 12981
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gfp cdc42  (Addgene inc)
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pcdna3  (Addgene inc)
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Plasmids containing BAR domain protein sequences used in this study.
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pcdna3 egfp cdc42 q61l  (Addgene inc)
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Plasmids containing BAR domain protein sequences used in this study.
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50mm tris hcl  (Thermo Fisher)
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Plasmids containing BAR domain protein sequences used in this study.
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cdc42 q61l  (Addgene inc)
91
Addgene inc cdc42 q61l
A . Western blot analysis of nuclear extracts from A549 cells cultured under serum-free conditions for 24 h and then stimulated with serum for the indicated time periods. B . MYC expression in nuclear extracts from A549 cells treated with the indicated inhibitors at 0.1 μM for 3d. For controls (BGT3D), cells treated with BGT and then released into drug-free medium for 3d are shown. C . MYC expression in nuclear extracts from KRAS WT and mutant NSCLC cell lines. D . MYC expression in nuclear extracts from CHAGO-K1 cells stably transduced with the activated or wild-type RAS isoforms. E . MYC expression in nuclear extracts from A549 cells stably transduced with GFP, PIK3CA H1047R (PIK3CAm), myrPDK1 (PDK1m), myrAKT (AKTm) and MYC T58A. F . MYC expression in nuclear extracts from HCC366 and CHAGO-K1 cells stably transduced with GFP, BRAF V600E (BRAFm), EGFRvIII (EGFRm), RAC1 G12V (RAC1m) and <t>CDC42</t> <t>Q61L</t> (CDC42m). G . A proposed mechanism of MYC regulation by oncogenic KRAS. H, I . MYC protein (H) and mRNA expression (I) in A549 cells treated with the indicated inhibitors for 3 days. Relative levels of MYC mRNA were normalized to the expression HPRT mRNA.
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cdc42 q61l  (Bio-Rad)
93
Bio-Rad cdc42 q61l
A . Western blot analysis of nuclear extracts from A549 cells cultured under serum-free conditions for 24 h and then stimulated with serum for the indicated time periods. B . MYC expression in nuclear extracts from A549 cells treated with the indicated inhibitors at 0.1 μM for 3d. For controls (BGT3D), cells treated with BGT and then released into drug-free medium for 3d are shown. C . MYC expression in nuclear extracts from KRAS WT and mutant NSCLC cell lines. D . MYC expression in nuclear extracts from CHAGO-K1 cells stably transduced with the activated or wild-type RAS isoforms. E . MYC expression in nuclear extracts from A549 cells stably transduced with GFP, PIK3CA H1047R (PIK3CAm), myrPDK1 (PDK1m), myrAKT (AKTm) and MYC T58A. F . MYC expression in nuclear extracts from HCC366 and CHAGO-K1 cells stably transduced with GFP, BRAF V600E (BRAFm), EGFRvIII (EGFRm), RAC1 G12V (RAC1m) and <t>CDC42</t> <t>Q61L</t> (CDC42m). G . A proposed mechanism of MYC regulation by oncogenic KRAS. H, I . MYC protein (H) and mRNA expression (I) in A549 cells treated with the indicated inhibitors for 3 days. Relative levels of MYC mRNA were normalized to the expression HPRT mRNA.
Cdc42 Q61l, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdc42-q61l plasmid  (GenScript corporation)
90
GenScript corporation cdc42-q61l plasmid
A . Western blot analysis of nuclear extracts from A549 cells cultured under serum-free conditions for 24 h and then stimulated with serum for the indicated time periods. B . MYC expression in nuclear extracts from A549 cells treated with the indicated inhibitors at 0.1 μM for 3d. For controls (BGT3D), cells treated with BGT and then released into drug-free medium for 3d are shown. C . MYC expression in nuclear extracts from KRAS WT and mutant NSCLC cell lines. D . MYC expression in nuclear extracts from CHAGO-K1 cells stably transduced with the activated or wild-type RAS isoforms. E . MYC expression in nuclear extracts from A549 cells stably transduced with GFP, PIK3CA H1047R (PIK3CAm), myrPDK1 (PDK1m), myrAKT (AKTm) and MYC T58A. F . MYC expression in nuclear extracts from HCC366 and CHAGO-K1 cells stably transduced with GFP, BRAF V600E (BRAFm), EGFRvIII (EGFRm), RAC1 G12V (RAC1m) and <t>CDC42</t> <t>Q61L</t> (CDC42m). G . A proposed mechanism of MYC regulation by oncogenic KRAS. H, I . MYC protein (H) and mRNA expression (I) in A549 cells treated with the indicated inhibitors for 3 days. Relative levels of MYC mRNA were normalized to the expression HPRT mRNA.
Cdc42 Q61l Plasmid, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Plasmids containing BAR domain protein sequences used in this study.

Journal: Science Advances

Article Title: Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling

doi: 10.1126/sciadv.ade1660

Figure Lengend Snippet: Plasmids containing BAR domain protein sequences used in this study.

Article Snippet: CDC42-Q61L , Human , pcDNA3 , Addgene #12600.

Techniques:

Other plasmids used in this study.

Journal: Science Advances

Article Title: Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling

doi: 10.1126/sciadv.ade1660

Figure Lengend Snippet: Other plasmids used in this study.

Article Snippet: CDC42-Q61L , Human , pcDNA3 , Addgene #12600.

Techniques:

A . Western blot analysis of nuclear extracts from A549 cells cultured under serum-free conditions for 24 h and then stimulated with serum for the indicated time periods. B . MYC expression in nuclear extracts from A549 cells treated with the indicated inhibitors at 0.1 μM for 3d. For controls (BGT3D), cells treated with BGT and then released into drug-free medium for 3d are shown. C . MYC expression in nuclear extracts from KRAS WT and mutant NSCLC cell lines. D . MYC expression in nuclear extracts from CHAGO-K1 cells stably transduced with the activated or wild-type RAS isoforms. E . MYC expression in nuclear extracts from A549 cells stably transduced with GFP, PIK3CA H1047R (PIK3CAm), myrPDK1 (PDK1m), myrAKT (AKTm) and MYC T58A. F . MYC expression in nuclear extracts from HCC366 and CHAGO-K1 cells stably transduced with GFP, BRAF V600E (BRAFm), EGFRvIII (EGFRm), RAC1 G12V (RAC1m) and CDC42 Q61L (CDC42m). G . A proposed mechanism of MYC regulation by oncogenic KRAS. H, I . MYC protein (H) and mRNA expression (I) in A549 cells treated with the indicated inhibitors for 3 days. Relative levels of MYC mRNA were normalized to the expression HPRT mRNA.

Journal: Oncotarget

Article Title: KRAS-dependent suppression of MYC enhances the sensitivity of cancer cells to cytotoxic agents

doi: 10.18632/oncotarget.14929

Figure Lengend Snippet: A . Western blot analysis of nuclear extracts from A549 cells cultured under serum-free conditions for 24 h and then stimulated with serum for the indicated time periods. B . MYC expression in nuclear extracts from A549 cells treated with the indicated inhibitors at 0.1 μM for 3d. For controls (BGT3D), cells treated with BGT and then released into drug-free medium for 3d are shown. C . MYC expression in nuclear extracts from KRAS WT and mutant NSCLC cell lines. D . MYC expression in nuclear extracts from CHAGO-K1 cells stably transduced with the activated or wild-type RAS isoforms. E . MYC expression in nuclear extracts from A549 cells stably transduced with GFP, PIK3CA H1047R (PIK3CAm), myrPDK1 (PDK1m), myrAKT (AKTm) and MYC T58A. F . MYC expression in nuclear extracts from HCC366 and CHAGO-K1 cells stably transduced with GFP, BRAF V600E (BRAFm), EGFRvIII (EGFRm), RAC1 G12V (RAC1m) and CDC42 Q61L (CDC42m). G . A proposed mechanism of MYC regulation by oncogenic KRAS. H, I . MYC protein (H) and mRNA expression (I) in A549 cells treated with the indicated inhibitors for 3 days. Relative levels of MYC mRNA were normalized to the expression HPRT mRNA.

Article Snippet: Retroviral vectors encoding HRAS, KRAS, NRAS, KRAS G12C, KRAS G12D, KRAS G12V, BRAF V600E, CDC42 Q61L, CRAF 22W, EGFRvIII, ERK2 R67S D321N, MEK1 C121S, MEK2 KW71, MEK2 K101A, MYCΔMBII, MYC T58A, shMYC, PIK3CA H1047R, myrAKT, myrPDK1, myrSGK1, RAC1 G12V and RALA Q75L were purchased from Addgene.

Techniques: Western Blot, Cell Culture, Expressing, Mutagenesis, Stable Transfection, Transduction